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Gene knockout cell lysates are the cell homogenate in RIPA buffer made with double-knockout cell lines. The Gene knockout cell lines are developed by CRISPR. The protein concentration was determined with BCA assay. A vial of lysate from the parental cell line was also included as an internal control.

https://www.creative-biogene.com/AK2-KO-Cell-Lysate-HEK293T-CLKO-0897-1404933-285.html
Dodał Marcia Brady

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